INTRODUCING THE COMPREHENSIVE H&E STAIN SYSTEM FROM AVANTIK

Consistent Staining. Crisp Delineation.

The Avantik Optik® H&E Staining System is a complementary set of quality reagents and stains designed to work in coordination with each other to produce consistent, conclusive results in your hematoxylin and eosin staining protocol which will surpass expectations in the lab every time.

The Optik family of H&E stains is the premier group of Avantik staining reagents designed to produce precise, consistent H&E staining with crisp delineation. The following are guidelines that will have an effective staining window of 2 to 5 minutes. Developed as progressive stains, the intensity of nuclear staining will increase as the time increases. The hematoxylin and eosin stains will have an expected throughput of 2,000-2,500 slides per 500mL bottle. Actual results may vary from lab to lab depending on the staining equipment used, control of carry over into each solution, and length of time stains are left exposed.

Optik Type 1 Recommended Staining Procedure

Station - Solution - Time

 1. Xylene* - 3 minutes

 2. Xylene* - 3 minutes

 3. Xylene* - 3 minutes

 4. 100% Ethanol - 1 minute

 5. 100% Ethanol - 1 minute

 6. 100% Ethanol - 1 minute

 7. 70% to 80% Ethanol - 1 minute

 8. Running H2OWash - 1 minute

 9. Optik Hematoxylin - 2-5 minutes

 10. Running H2O Wash - 3 minutes

 11. Optik Clarifier - 30-90 seconds

 12. Running H2OWash - 1 minute

 13. Optik Bluing Solution - 1 minute

 14. Running H2OWash - 1 minute

 15. 70% to 80% Ethanol - 45 seconds

 16. Optik Eosin - 30-90 seconds

 17. 100% Ethanol - 1 minute

 18. 100% Ethanol - 1 minute

 19. 100% Ethanol - 1 minute

 20. Xylene* - 1 minute

 21. Xylene* - 1 minute

 22. Xylene* - 1 minute

 

Final Step: Mount and Coverslip with Covermount

* Ultraclear may be used in place of Xylene.

Times in Xylene substitutes may need to be extended. 

 

The hematoxylin and eosin stains can have an expected throughput of 2,000-2,500 slides per 500mL bottle. Actual results may vary from lab to lab depending on the staining equipment used, control of carry over into each solution, and length of time stains are left exposed. As a general rule, we recommend changing the hematoxylin and eosin stains once per week if throughput has not been reached. Clarifying and Bluing solutions should be changed more often.

 

Hematoxylin - Item# RS4358-A - 500mL Bottle

Eosin - Y - Item# RS4359-A - 500mL Bottle

Bluing Reagent (Concentrate) - Item# RS4362-A - 500mL Bottle  - 20x concentration. Yields about 10L of complete solution.

Bluing Reagent - Item# RS4363-B - 1 Gallon Bottle

Aqueous Clarifier (Concentrate) - Item# RS4360-A - 500mL Bottle - 20x concentration. Yields about 10L of complete solution.

Aqueous Clarifier - Item# RS4361-B - 1 Gallon Bottle

 

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